The distal tubule becomes convoluted and eventually empties into a collecting
tubule. The cells of the distal convoluted tubule are cuboidal. In contrast to cells of the
PCT, cells of the DCT are smaller, have less cytoplasm and hence appear more
basophilic, and they have no brush border (B-67, H&E [10x, 20x, 40x-labeled]; B-68, PAS [10x, 20x, 40x-labeled]; B-69, PAS [10x, 20x, 40x-labeled]; B-71, H&E [10x, 20x, 40x-labeled]). Also, DCTs are smaller than proximal tubules (see R-P, Fig. 20.18 & Pl. 72). Look for DCTs
in slides B-66 through B-71. Since the DCTs lack a brush border, they will be more readily distinguished from PCTs in sections stained with PAS.
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